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The expression of MDA in nmol/mg when plotted in a Mann Whitney U test bar graph showed no change in the DCM group compared to the HC

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Primary Tumor Growth Spontaneous Metastases Cell lines derived from 4T1 primary tumors (see above) were stably transduced with pLKO.1 vectors (Control and pooled shRNAs targeting Gstt1 ), harvested by trypsinization, washed twice in PBS, resuspended at 1 x 10 4 in 90ul PBS and 10% Matrigel (Corning) and injected into the 4 th inguinal mammary fat pad of BALB/c mice (Jackson Laboratory) to establish mammary tumors