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Concentration = Total peptide (mg) / Total water added (mL) For a 30 mg vial: Add 1 mL = 30 mg/mL (very concentrated) Add 2 mL = 15 mg/mL (concentrated) Add 3 mL = 10 mg/mL (standard, recommended) Add 4 mL = 7.5 mg/mL (dilute) Add 5 mL = 6 mg/mL (very dilute) Add 6 mL = 5 mg/mL (most dilute practical option) Each of these concentrations works