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Aside from histological methods, TJPs can be estimated by assessing the protein expression using the Western blot technique, as documented in a human trial of patients with intestinal barrier abnormalities [48], or by evaluating the mRNA levels of TJPs by RT-qPCR, as has been assayed in colonic tissues from healthy human volunteers [49]

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Positive effects are plausible enough the US Anti-doping Agency has banned MOTS-c as a PED due to its role in AMPK pathway modulation, however, no causal evidence of positive effects of MOTS-c in healthy humans is available