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Key findings from receptor studies [2] : Long-term studies in β₃-AR knockout mice showed no body weight reduction or lipolysis increases This verified the receptor pathway’s importance for sustained metabolic effects Acute experiments showed AOD9604 increased energy expenditure even in knockout mice Results suggest β₃-AR expression enhances sensitivity but additional mechanisms exist beyond the direct receptor activation cAMP-PKA-HSL Pathway Activation The peptide triggers increases in intracellular cyclic AMP (cAMP) within adipocytes

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Endogenous GHRH is a 44-amino acid polypeptide secreted by the arcuate nucleus of the hypothalamus