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We then filtered for peptides derived from differentially expressed parent genes as determined from RNA-sequencing (RNA-seq) data from 153 neuroblastoma tumours compared with 1,641 normal tissues (from the TARGET 21 and GTEx 17 databases, respectively), resulting in 61 peptides derived from genes with mRNA expression one log-fold higher in tumour for each normal tissue ( P 10 11 diversity) was constructed using fully-germline IGLV3-1 and IGLV6-57 scaffolds paired with the IGHV3-23 scaffold as described 33 , with fully synthetic amino acid diversity in both V L and V H CDR3 loops
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